Journal: Investigative Ophthalmology & Visual Science

Article Title: Single-Cell Characterization of the Frizzled 5 ( Fz5 ) Mutant Mouse and Human Persistent Fetal Vasculature (PFV)

doi: 10.1167/iovs.64.3.8

Figure Lengend Snippet: Molecular profiles of the P6 remaining clusters. ( A–C ) Transmission electron microscopy of the adult mutant pigmentary vitreous showing the existing PFV cell types. Peri, pericyte process; EC, endothelial process; Mac, macrophage; Fibro, fibroblasts; Mela, melanocytes ( dashed lines ). Vessels are indicated by short lines. Arrows point to collagen fibers. ( D–F ) Expression of Col1a1 ( red ) and CD31 ( green ) in the vitreous tissues detected by immunohistochemistry. The arrows in the mutant vitreous retrolental mass. The boxed area in ( E ) is magnified as ( F ). V, vitreous. ( G ) The remaining P6 mutant clusters on UMAPs. C1, endothelial; C2 & C9, fibroblasts; C4 & C13, pericytes. ( H ) GO terms of C1 selected from top items with P value < 0.001. ( I ) GO terms of C2 selected from top items with P value <6e-10. ( J ) GO terms of C9 selected from top items with P value <4e-5. ( K ) GO terms of C4 selected from top items with P value <5e-14. ( L ) GO terms of C13 selected from top items with P value <4e-5. ( M ) GO terms of C29 selected from top items with P value <2e-12. The boxed items highlight features consistent with cluster survival or regression. Note that “gene ratio” was left out from the GO items for conveniently viewing the data.

Article Snippet: The primary antibodies used for this study were rabbit anti-Col1a1 (1:500, A1352; ABclonal Technology, Woburn, MA, USA), rat anti-CD31 (1:500, 557355; BD Bioscience), rabbit anti- AP2β (1:200, 2509S; Cell Signaling Technology, Danvers, MA, USA), mouse anti-alkaline phosphatase (1:100, 14-9870-82; Invitrogen, Carlsbad, CA, USA), rabbit anti-Pax3 (1:100, bs-1097R; Bioss Antibodies Inc., Woburn, MA, USA), goat anti-Sox10 (1:20, AF2864; R&D Systems, Minneapolis, MN, USA), and mouse anti-Tyrp1 (1:100, ab3312; Abcam, Cambridge, MA, USA).

Techniques: Transmission Assay, Electron Microscopy, Mutagenesis, Expressing, Immunohistochemistry